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KMID : 1098420090170060445
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Korean Journal of Medicinal Crop Science
2009 Volume.17 No. 6 p.445 ~ p.451
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Cultivar Discrimination of Korean and Chinese Boxthorn (Lycium chinese Mill. And Lycium barbarum L.) using SSR Markers
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Chung Jong-Wook
Lee Gi-An
Lee Sox-Su
Bang Kyong-Hwan
Park Yong-Jin
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Abstract
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This study was undertaken to develop a technique of discrimination using SSR makers in boxthorn cultivars. Forty one boxthorn cultivars, which were collected from Korea and China, were evaluated by 10 SSR markers. Total of 61 alleles were detected, ranging from 3 to 13 with an average of 6.1 alleles per locus. The averages of gene diversity and PIC values were 0.482 and 0.428, with a range from 0.25 (GB-LCM-022 and GB-LCM-087) to 0.83 (GB-LCM-167) and from 0.24 (GB-LCM-022 and GB-LCM-087) to 0.81 (GB-LCM-167), respectively. Five markers out of 10 markers, GB-LCM- 022, GB-LCM-075, GB-LCM-104, GB-LCM-167 and GB-LCM-217, were selected as key markers for discrimination in boxthorn cultivars. All of boxthorn cultivars were individually distinguished by the combination of five SSR markers.
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KEYWORD
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SSRs, Simple Sequence Repeats, cultivar Discrimination, Boxthorn, Lycium chinense Mill., Lycium barbrum L.
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